Isolation and characterization of Klebsiella pneumoniae specific bacteriophages from sewage samples
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Animal Structures virology MeSH
- Bacteriophages classification isolation & purification physiology ultrastructure MeSH
- DNA Fingerprinting MeSH
- DNA, Viral genetics MeSH
- DNA genetics MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Genetic Variation MeSH
- Genotype MeSH
- Klebsiella pneumoniae virology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Sewage virology MeSH
- Podoviridae classification isolation & purification physiology ultrastructure MeSH
- Virus Attachment MeSH
- Random Amplified Polymorphic DNA Technique MeSH
- Microscopy, Electron, Transmission MeSH
- Virion ultrastructure MeSH
- Viral Proteins analysis MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Viral MeSH
- DNA MeSH
- Sewage MeSH
- Viral Proteins MeSH
Five bacteriophages (Kpn5, Kpn12, Kpn13, Kpn17 and Kpn22), each having specificity against Klebsiella pneumoniae strain B5055, were isolated from sewage samples and characterized in terms of growth characteristics, genetic material, morphology and structural proteins. Adsorption rate as well as single step growth curve experiments showed variation among phages. Restriction enzyme digestion of DNA confirmed the presence of double stranded DNA as well as the heterogeneous nature of genetic material. RAPD-PCR was performed to further distinguish these closely related phages. Their genome fingerprint confirmed their diversity. Transmission electron microscopy, on the other hand, showed their morphological similarity; they were assigned to family Podoviridae, order Caudovirales on the basis of their head and tail morphology. Structural proteins resolved on SDS-PAGE showed the presence of similar major outer membrane proteins. The bacteriophages, belonging to Podoviridae family with short stumpy tails, were found to be nontoxic to mice. They showed maximum count in various organs at 6 h post inoculation, which persisted till 36 h. These phages thus have the potential to be used for phage therapy.
Department of Microbiology Panjab University Basic Medical Sciences Building Chandigarh 160014 India
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