Isolation and characterization of Klebsiella pneumoniae specific bacteriophages from sewage samples
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- anatomické struktury zvířat virologie MeSH
- bakteriofágy klasifikace izolace a purifikace fyziologie ultrastruktura MeSH
- DNA fingerprinting MeSH
- DNA virů genetika MeSH
- DNA genetika MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- genetická variace MeSH
- genotyp MeSH
- Klebsiella pneumoniae virologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- odpadní vody virologie MeSH
- Podoviridae klasifikace izolace a purifikace fyziologie ultrastruktura MeSH
- přichycení viru MeSH
- technika náhodné amplifikace polymorfní DNA MeSH
- transmisní elektronová mikroskopie MeSH
- virion ultrastruktura MeSH
- virové proteiny analýza MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA virů MeSH
- DNA MeSH
- odpadní vody MeSH
- virové proteiny MeSH
Five bacteriophages (Kpn5, Kpn12, Kpn13, Kpn17 and Kpn22), each having specificity against Klebsiella pneumoniae strain B5055, were isolated from sewage samples and characterized in terms of growth characteristics, genetic material, morphology and structural proteins. Adsorption rate as well as single step growth curve experiments showed variation among phages. Restriction enzyme digestion of DNA confirmed the presence of double stranded DNA as well as the heterogeneous nature of genetic material. RAPD-PCR was performed to further distinguish these closely related phages. Their genome fingerprint confirmed their diversity. Transmission electron microscopy, on the other hand, showed their morphological similarity; they were assigned to family Podoviridae, order Caudovirales on the basis of their head and tail morphology. Structural proteins resolved on SDS-PAGE showed the presence of similar major outer membrane proteins. The bacteriophages, belonging to Podoviridae family with short stumpy tails, were found to be nontoxic to mice. They showed maximum count in various organs at 6 h post inoculation, which persisted till 36 h. These phages thus have the potential to be used for phage therapy.
Department of Microbiology Panjab University Basic Medical Sciences Building Chandigarh 160014 India
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