Measurement of cellular excitability by whole cell patch clamp technique
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
20626213
DOI
10.33549/physiolres.932000
PII: 932000
Knihovny.cz E-resources
- MeSH
- Action Potentials MeSH
- Models, Biological MeSH
- Cell Membrane metabolism MeSH
- Ion Channel Gating MeSH
- Ion Channels metabolism MeSH
- Humans MeSH
- Membrane Potentials MeSH
- Patch-Clamp Techniques * MeSH
- Calcium Signaling MeSH
- Calcium Channels, T-Type metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Ion Channels MeSH
- Calcium Channels, T-Type MeSH
Patch clamp method developed more than 30 years ago is widely used for investigation of cellular excitability manifested as transmembrane ionic current and/or generation of action potentials. This technique could be applied to measurement of ionic currents flowing through individual (single) ion channels or through the whole assembly of ion channels expressed in the whole cell. Whole cell configuration is more common for measurement of ion currents and the only one enabling measurement of action potentials. This method allows detailed analysis of mechanisms and structural determinants of voltage-dependent gating of ion channels as well as regulation of channel activity by intracellular signaling pathways and pharmacological agents.
Institute of Molecular Physiology and Genetics Slovak Academy of Sciences Bratislava Slovak Republic
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