Fructanolytic and saccharolytic enzymes of the rumen bacterium Pseudobutyrivibrio ruminis strain 3--preliminary study
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Rumen microbiology MeSH
- Bacterial Proteins isolation & purification metabolism MeSH
- Chromatography MeSH
- DNA, Bacterial chemistry genetics MeSH
- Enzymes isolation & purification metabolism MeSH
- Fructans metabolism MeSH
- Phylogeny MeSH
- Gram-Positive Bacteria classification enzymology genetics isolation & purification MeSH
- Inulin metabolism MeSH
- Molecular Sequence Data MeSH
- Phleum chemistry MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sucrose metabolism MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- Enzymes MeSH
- Fructans MeSH
- Inulin MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
- Sucrose MeSH
P. ruminis strain 3 was isolated from the ovine rumen and identified on the basis of comparison of its 16S rRNA gene with GenBank. The bacterium was able to grow on Timothy grass fructan, inulin, sucrose, fructose and glucose as a sole carbon source, reaching absorbance of population in a range of 0.4-1.2. During 1 d the bacteria exhausted 92-97% of initial dose of saccharides except for inulin (its utilization did not exceed 33%). The bacterial cell extract catalyzed the degradation of Timothy grass fructan, inulin and sucrose in relation to carbon source present in growth medium. Molecular filtration on Sephadex G-150, polyacrylamide gel electrophoresis combined with zymography technique and TLC was used to identify enzymes responsible for the digestion of sucrose and both polymers of fructose. Two specific endolevanases (EC 3.2.1.65), nonspecific beta-fructofuranosidase (EC 3.2.1.80 and/or EC 3.2.1.26) and sucrose phosphorylase (EC 2.4.1.7) were detected in cell-free extract from bacteria grown on Timothy grass fructan.
See more in PubMed
J Appl Microbiol. 2002;92(1):140-6 PubMed
Anal Biochem. 1969 Mar;27(3):545-54 PubMed
GENBANK
EU714406
