Study of electromigration effects on a pH boundary during the on-line electrokinetic preconcentration by capillary electrophoresis
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Electric Conductivity MeSH
- Electrophoresis, Capillary methods MeSH
- Electrolytes isolation & purification MeSH
- Capillary Action MeSH
- Hydrogen-Ion Concentration MeSH
- Benzoic Acid chemistry isolation & purification MeSH
- Boric Acids chemistry MeSH
- Phosphoric Acids chemistry MeSH
- Methanol MeSH
- Online Systems MeSH
- Organic Chemicals chemistry MeSH
- Osmolar Concentration MeSH
- Silicon Dioxide MeSH
- Solvents chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- boric acid MeSH Browser
- Electrolytes MeSH
- Benzoic Acid MeSH
- Boric Acids MeSH
- Phosphoric Acids MeSH
- Methanol MeSH
- Organic Chemicals MeSH
- Silicon Dioxide MeSH
- phosphoric acid MeSH Browser
- Solvents MeSH
A contribution to the description of electrokinetic effects on the pH boundary formed by sodium borate pH 9.5 and sodium phosphate pH 2.5 electrolytes for on-line preconcentration of weak acids is presented in this article. Simulations of electrokinetic injections together with experimental studies using contactless conductivity detection verified that the preconcentration is induced mainly by dissociation changes of analytes on the pH boundary and transient ITP state. Moreover, a study of the addition of organic solvent to the injection electrolyte was performed with impressive results. Subnanomolar LODs of hydroxybenzoic acids were achieved with 80% of methanol in the injection electrolyte which represents more than 70 000-fold preconcentration in comparison with classical CZE method.
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