Imaging of Toxocara canis larvae labelled by CFSE in BALB/c mice
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Animal Structures parasitology MeSH
- Staining and Labeling MeSH
- Time Factors MeSH
- Fluoresceins metabolism MeSH
- Fluorescent Dyes metabolism MeSH
- Disease Models, Animal MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Rodent Diseases parasitology MeSH
- Succinimides metabolism MeSH
- Toxocara canis growth & development isolation & purification pathogenicity MeSH
- Toxocariasis parasitology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 5-(6)-carboxyfluorescein diacetate succinimidyl ester MeSH Browser
- Fluoresceins MeSH
- Fluorescent Dyes MeSH
- Succinimides MeSH
Mice are used most often as a model for human toxocariasis caused by Toxocara canis larvae. Variety of symptoms developing during the infection reflects behaviour of the larvae, which are able to escape from the intestine and further invade and damage various host organs. In order to find an approach enabling observation on parasite behaviour in mouse in vivo, we used an epifluorescence method and a small animal imaging system (SAIS). Larvae of T. canis were labelled by carboxyfluorescein succinimidyl ester (CFSE) which incorporated on the parasite gastrointestinal tract. Following infection of BALB/c mice by CFSE-labelled larvae it has been observed that staining had no influence on viability and further migratory activity of the parasites through the host organs (the intestine, liver, lungs and brain) where they were detected by SAIS until day 17 p.i. In addition, the dye did not affect larval antigenic activity as well as the development of related immune response. Imaging of parasites labelled by CFSE, therefore, may represent a promising way to study behaviour of T. canis larvae in a paratenic host.
See more in PubMed
Int J Parasitol. 1983 Oct;13(5):517-21 PubMed
J Infect Dis. 1990 Aug;162(2):571 PubMed
Am J Trop Med Hyg. 2004 Aug;71(2):216-21 PubMed
Crit Rev Microbiol. 1997;23(3):233-51 PubMed
J Helminthol. 2001 Jun;75(2):175-81 PubMed
Vet Parasitol. 2008 Mar 25;152(1-2):85-93 PubMed
Parasitology. 1969 Nov;59(4):837-41 PubMed
Trans R Soc Trop Med Hyg. 1971;65(4):450-3 PubMed
Brain Res Bull. 1983 Apr;10(4):547-50 PubMed
J Immunol Methods. 1994 May 2;171(1):131-7 PubMed
Kisaengchunghak Chapchi. 1976 Jun;14(1):51-60 PubMed
J Helminthol. 1997 Sep;71(3):253-5 PubMed
Rev Inst Med Trop Sao Paulo. 2007 Sep-Oct;49(5):279-87 PubMed
J Parasitol. 1961 Aug;47:652-6 PubMed
Vet Parasitol. 2004 May 7;121(1-2):115-24 PubMed
J Helminthol. 1994 Dec;68(4):359-60 PubMed
J Infect Dis. 1952 Mar-Apr;90(2):165-76 PubMed
Epidemiol Rev. 1981;3:230-50 PubMed
Parasitology. 1958 May;48(1-2):184-209 PubMed
J Infect Dis. 1953 Mar-Apr;92(2):114-7 PubMed
Clin Infect Dis. 1992 Oct;15(4):743-4 PubMed
Parasitol Int. 2008 Dec;57(4):525-9 PubMed
Acta Trop. 2008 Feb;105(2):124-30 PubMed
Parasitology. 1995 Jun;110 ( Pt 5):535-45 PubMed
J Helminthol. 1969;43(3):267-72 PubMed
Immunol Cell Biol. 1999 Dec;77(6):499-508 PubMed
J Infect Dis. 1989 Oct;160(4):735-6 PubMed
Z Parasitenkd. 1968;30(2):152-61 PubMed
J Parasitol. 1975 Aug;61(4):781-2 PubMed
Korean J Parasitol. 2001 Mar;39(1):1-11 PubMed
Vet Parasitol. 1984 Dec;17(1):75-83 PubMed
Vet Parasitol. 1984 Dec;17(1):65-73 PubMed
Vet Parasitol. 2005 Nov 25;134(1-2):121-30 PubMed
Toxocara canis larvae reinfecting BALB/c mice exhibit accelerated speed of migration to the host CNS
