Using a small animal imaging system, migratory activity of Toxocara canis larvae stained by carboxyfluorescein succinimidyl ester (CFSE) was observed post primary infection (PPI) and post reinfection (PR) of BALB/c mice. Each infection was performed with 1,000 larvae per mouse. Primary infections were performed with labeled larvae, while for challenge infections the reinfecting larvae were stained by CFSE. The worm burden in mouse organs was determined during a period from 6 h to 21 days and 4 months PPI and PR. In comparison with primary infections that led to the first larvae appearance in the brain after 60 h, greatly accelerated migration of the parasites administered 3 weeks PPI to the CNS and eyes of challenged mice was noted-in both organs the larvae appeared 6 h PR. In all challenged mice, reinfecting larvae prevailed in the resident parasite population. Preliminary experiments with Toxocara cati larvae also revealed early brain involvement in primarily infected mice. Staining of T. canis larvae by CFSE had no effect on the development of a humoral antibody response against T. canis excretory-secretory antigens. In ELISA, elevated levels of specific IgG and IgG1 were noted on day 14 PPI and the levels of antibodies increased till the end of experiment. Reinfection induced an increase in the levels of both antibodies. In terms of optical density, IgG1 antibodies gave higher values in all sera examined. In ELISA for IgG antibodies, an increase in the avidity index of around 50% was detected 1 month PPI; higher-avidity antibodies were also detected in sera of reinfected animals.

National Reference Laboratory for Tissue Helminthoses General University Hospital Prague Prague Czech Republic

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Ann Trop Med Parasitol. 1987 Dec;81(6):673-9 PubMed

Am J Trop Med Hyg. 1976 Jul;25(4):573-80 PubMed

Vet Parasitol. 1989 Oct;33(3-4):297-307 PubMed

Br J Ophthalmol. 1984 Feb;68(2):89-96 PubMed

Rev Soc Bras Med Trop. 2004 Mar-Apr;37(2):169-74 PubMed

Brain Res. 2001 Mar 30;896(1-2):86-95 PubMed

Lancet Neurol. 2008 Jan;7(1):84-96 PubMed

Parasitology. 1956 May;46(1-2):54-78 PubMed

Mem Inst Oswaldo Cruz. 2005 Mar;100 Suppl 1:73-81 PubMed

Am J Trop Med Hyg. 1989 Sep;41(3 Suppl):21-34 PubMed

J Helminthol. 1997 Sep;71(3):253-5 PubMed

Parasite Immunol. 2008 Mar;30(3):187-90 PubMed

Zoonoses Public Health. 2009 May;56(4):198-205 PubMed

Wiad Parazytol. 2004;50(2):229-36 PubMed

Int J Parasitol. 1983 Oct;13(5):517-21 PubMed

Parasitology. 1955 May;45(1-2):41-55 PubMed

Infect Immun. 1985 Jun;48(3):697-703 PubMed

Parasitol Res. 1994;80(8):691-5 PubMed

Exp Parasitol. 2007 Jan;115(1):76-82 PubMed

Mol Biochem Parasitol. 2010 Jul;172(1):1-8 PubMed

Parasitol Res. 1995;81(1):13-7 PubMed

Rev Inst Med Trop Sao Paulo. 2007 Sep-Oct;49(5):279-87 PubMed

Genet Mol Res. 2008 May 20;7(2):444-50 PubMed

Vet Parasitol. 2004 May 7;121(1-2):115-24 PubMed

J Helminthol. 2001 Mar;75(1):33-41 PubMed

Allergy Asthma Immunol Res. 2010 Oct;2(4):267-70 PubMed

J Helminthol. 1994 Dec;68(4):359-60 PubMed

Epidemiol Rev. 1981;3:230-50 PubMed

Acta Trop. 2008 May;106(2):90-5 PubMed

J Helminthol. 1983 Jun;57(2):95-9 PubMed

Clin Microbiol Rev. 2003 Apr;16(2):265-72 PubMed

Am J Trop Med Hyg. 1987 Jan;36(1):75-82 PubMed

Vet Parasitol. 1991 May;38(4):289-98 PubMed

Chin J Physiol. 2004 Dec 31;47(4):189-96 PubMed

J Helminthol. 2001 Jun;75(2):175-81 PubMed

Nat Rev Neurosci. 2006 Jan;7(1):41-53 PubMed

Parasitol Int. 2010 Dec;59(4):491-6 PubMed

Vet Parasitol. 2003 Apr 18;113(2):145-55 PubMed

Parasite Immunol. 2008 Oct;30(10):525-34 PubMed

Cesk Slov Oftalmol. 2002 Apr;58(2):75-83 PubMed

Acta Trop. 2008 Feb;105(2):124-30 PubMed

Nihon Juigaku Zasshi. 1968 Jun;30(3):151-66 PubMed

Epidemiol Mikrobiol Imunol. 2001 Apr;50(2):67-70 PubMed

Parasitology. 2006 Jun;132(Pt 6):791-801 PubMed

J Helminthol. 1969;43(3):267-72 PubMed

J Infect Dis. 1989 Oct;160(4):735-6 PubMed

Int J Parasitol. 1990 Apr;20(2):263-4 PubMed

Rev Med Chil. 2010 Apr;138(4):483-6 PubMed

Z Parasitenkd. 1968;30(2):152-61 PubMed

J Parasitol. 1975 Aug;61(4):781-2 PubMed

Neurology. 2000 Jul 12;55(1):104-11 PubMed

Parasitology. 2008 May;135(6):725-33 PubMed

Korean J Parasitol. 2001 Mar;39(1):1-11 PubMed

Childs Nerv Syst. 2004 Jul;20(7):468-72 PubMed

Vet Parasitol. 1984 Dec;17(1):75-83 PubMed

Folia Parasitol (Praha). 1982;29(4):309-13 PubMed

Int J Parasitol. 1976 Jun;6(3):247-51 PubMed

J Helminthol. 2001 Mar;75(1):23-32 PubMed

Parasitol Res. 2011 Apr;108(4):1007-14 PubMed

Parasite Immunol. 1998 Jul;20(7):311-7 PubMed

Vet Parasitol. 1984 Dec;17(1):65-73 PubMed

Acta Trop. 2006 Feb;97(2):204-11 PubMed

Vet Parasitol. 2005 Nov 25;134(1-2):121-30 PubMed

Int J Parasitol. 1992 Nov;22(7):1037-8 PubMed

Parasite Immunol. 2008 Mar;30(3):181-5 PubMed

Parasitology. 1998 Jun;116 ( Pt 6):579-94 PubMed

Neurobehavioral Disorders and Pathological Changes in the Brain of Mice Are Caused by Chronic Toxocara canis Larval Invasion with Low to Moderate Inoculum