Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21198185
DOI
10.1117/1.3509112
Knihovny.cz E-resources
- MeSH
- Chondrocytes cytology metabolism transplantation MeSH
- Microscopy, Fluorescence methods MeSH
- Image Interpretation, Computer-Assisted methods MeSH
- Collagen metabolism ultrastructure MeSH
- Rabbits MeSH
- Cells, Cultured MeSH
- Nonlinear Dynamics MeSH
- Tissue Scaffolds * MeSH
- Imaging, Three-Dimensional methods MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Collagen MeSH
Extracellularly distributed collagen and chondrocytes seeded in gelatine and poly-ɛ-caprolactone scaffolds are visualized by two-photon excitation microscopy (TPEM) and second-harmonic generation (SHG) imaging in both forward and backward nondescanned modes. Joint application of TPEM and SHG imaging in combination with stereological measurements of collagen enables us not only to take high-resolution 3-D images, but also to quantitatively analyze the collagen volume and a spatial arrangement of cell-collagen-scaffold systems, which was previously impossible. This novel approach represents a powerful tool for the analysis of collagen-containing scaffolds with applications in cartilage tissue engineering.
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