IncN plasmids carrying bla CTX-M-1 in Escherichia coli isolates on a dairy farm
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
21276666
DOI
10.1016/j.vetmic.2010.11.032
PII: S0378-1135(10)00572-9
Knihovny.cz E-resources
- MeSH
- beta-Lactamases genetics MeSH
- Cephalosporins pharmacology MeSH
- DNA, Bacterial genetics MeSH
- Escherichia coli classification genetics isolation & purification MeSH
- Escherichia coli Infections microbiology veterinary MeSH
- Cattle Diseases microbiology MeSH
- Plasmids genetics MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Gene Transfer, Horizontal MeSH
- Escherichia coli Proteins genetics MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Cephalosporin Resistance MeSH
- Cattle microbiology MeSH
- Animals MeSH
- Check Tag
- Cattle microbiology MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- beta-lactamase TEM-3 MeSH Browser
- beta-Lactamases MeSH
- Cephalosporins MeSH
- DNA, Bacterial MeSH
- Escherichia coli Proteins MeSH
The aim of the study was to compare the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli bovine isolates on a conventional dairy cattle farm with high consumption of parenteral and intramammary cephalosporins (farm A) and on an organic dairy farm with no cephalosporin use (farm B). ESBL-producing E. coli were isolated from rectal swabs and milk filters by selective cultivation on MacConkey agar with cefotaxime (2mg/l). ESBL genes were identified by polymerase chain reaction (PCR) and sequencing, and the genetic diversity of the isolates was determined by XbaI pulsed field gel electrophoresis (PFGE). Conjugative transfer, incompatibility group, and restriction fragment length polymorphism (RFLP) profiles of the ESBL-carrying plasmids were studied. Higher prevalence (39%, n(rectal samples in cows)=309) of CTX-M-1-producing E. coli isolates was found on farm A compared to farm B (<1%, n(rectal samples in cows)=154; 0%, n(rectal samples in calves)=46). Using PFGE, the isolates from farm A were divided into nine pulsotypes. In all ESBL-positive isolates, the bla(CTX-M-1) gene was carried on 40 kb IncN conjugative plasmids of three related HincII restriction profiles. Horizontal gene transfer through transmission of IncN plasmids harboring bla(CTX-M-1) as well as clonal dissemination of a particular clone seems to be involved in dissemination of CTX-M-1-producing E. coli isolates in cows on the farm using cephalosporins in treating bacterial infections. The study demonstrates a possible role of cephalosporin use in the widespread occurrence of CTX-M-1-producing E. coli on the conventional dairy cattle farm compared to the organic farm.
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