Biosynthesis of ω-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- buněčná membrána metabolismus MeSH
- difenylhexatrien metabolismus MeSH
- fluidita membrány * MeSH
- fluorescenční barviva metabolismus MeSH
- fluorescenční polarizace MeSH
- Geobacillus stearothermophilus metabolismus MeSH
- gramnegativní bakterie klasifikace metabolismus MeSH
- kyseliny karboxylové metabolismus MeSH
- nenasycené mastné kyseliny biosyntéza MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- difenylhexatrien MeSH
- fluorescenční barviva MeSH
- kyseliny karboxylové MeSH
- nenasycené mastné kyseliny MeSH
Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize ω-alicyclic fatty acids (ω-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize ω-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FA composition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of ω-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of ω-FAs not found in the natural environment of Geobacillus and Meiothermus strains.
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