Capillary electrophoresis immunoassays with conjugated quantum dots
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Electrophoresis, Capillary methods MeSH
- Immunoassay methods MeSH
- Quantum Dots * MeSH
- Luminescent Measurements MeSH
- Ovalbumin chemistry MeSH
- Cadmium Compounds chemistry MeSH
- Tellurium chemistry MeSH
- Particle Size MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- cadmium telluride MeSH Browser
- Ovalbumin MeSH
- Cadmium Compounds MeSH
- Tellurium MeSH
Water-soluble CdTe quantum dots (QDs) and their conjugates with antibodies and antigenes were prepared by optimized procedures for applications in CE immunoassays. The QD size of 3.5 nm, excitation spectrum in the range of 300-500 nm, the maximum wavelength of the emission spectrum at 610 nm, quantum yield of 0.25 and luminescence lifetimes in the range of 3.6-43 ns were determined. The 0.1 M solution of TRIS/TAPS (pH 8.3) was found to be the optimum buffer for the separation of the antiovalbumin-ovalbumin immunocomplex from the free conjugates of QDs.
References provided by Crossref.org
Time-Dependent Growth of Silica Shells on CdTe Quantum Dots
