Selection and differentiation of Bacillus spp. Antagonistic to Fusarium oxysporum f.sp. lycopersici and Alternaria solani infecting Tomato
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Alternaria růst a vývoj patogenita MeSH
- antibióza * MeSH
- Bacillus klasifikace izolace a purifikace metabolismus fyziologie MeSH
- biologická kontrola škůdců metody MeSH
- biomasa MeSH
- chitinasy metabolismus MeSH
- DNA bakterií genetika MeSH
- Fusarium růst a vývoj patogenita MeSH
- glukan-1,3-beta-glukosidasa metabolismus MeSH
- kořeny rostlin mikrobiologie MeSH
- listy rostlin mikrobiologie MeSH
- molekulární typizace MeSH
- nemoci rostlin mikrobiologie MeSH
- siderofory metabolismus MeSH
- Solanum lycopersicum růst a vývoj mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- beta-1,3-exoglucanase MeSH Prohlížeč
- chitinasy MeSH
- DNA bakterií MeSH
- glukan-1,3-beta-glukosidasa MeSH
- siderofory MeSH
Antagonistic Bacillus spp. displaying in vitro production of siderophore, chitinase, and β-1,3-glucanase were identified from dual culture assays. In independent greenhouse studies, seed bacterization and soil application of Bacillus atrophaeus S2BC-2 challenge inoculated with Fusarium oxysporum f.sp. lycopersici (FOL) and Alternaria solani (AS) recorded low percent disease index of 25.3 and 28.7, respectively, over nonbacterised pathogen control (44.3 and 56.4). The low disease incidence corroborated with tomato growth promotion with high vigor index (8,041.2) and fresh plant weight (82.5 g) on challenge inoculation with FOL. Analysis of root and leaf samples in rhizobacterial treatment challenged with FOL and AS revealed maximum induction of chitinase (1.9 and 1.7 U/mg of protein, respectively) and β-1,3-glucanase (23.5 and 19.2 U/mg of protein, respectively). In native gel activity assays, the rhizobacterial treatment on challenge inoculation strongly expressed three high intensity PO isoforms along with one low intensity isoform. In studies on genetic diversity of the Bacillus strains by repetitive extragenomic palindromic-polymerase chain reaction (REP-PCR) and amplified rDNA restriction analysis (ARDRA) patterns, ARDRA was more highly discriminant than REP-PCR and allowed grouping of the strains and differentiation of the antagonistic strains from other isolates.
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