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Selection and differentiation of Bacillus spp. Antagonistic to Fusarium oxysporum f.sp. lycopersici and Alternaria solani infecting Tomato

Shanmugam, Veerubommu
Author Shanmugam, Veerubommu Floriculture Pathology Laboratory, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, 176 061, Himachal Pradesh, India. shanpatho@yahoo.com
Atri, Kamini
Author Atri, Kamini
Gupta, Samriti
Author Gupta, Samriti
Kanoujia, Nandina
Author Kanoujia, Nandina
Naruka, Digvijay Singh
Author Naruka, Digvijay Singh

Folia microbiologica. 2011 Mar ; 56 (2) : 170-7. [epub] 20110419

Folia Microbiol (Praha)
ISSN 1874-9356 | 0015-5632
Source

Language English Country United States Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid21503737

PubMed 21503737
DOI 10.1007/s12223-011-0031-3
Knihovny.cz E-resources

MeSH
Alternaria growth & development pathogenicity MeSH
Antibiosis * MeSH
Bacillus classification isolation & purification metabolism physiology MeSH
Pest Control, Biological methods MeSH
Biomass MeSH
Chitinases metabolism MeSH
DNA, Bacterial genetics MeSH
Fusarium growth & development pathogenicity MeSH
Glucan 1,3-beta-Glucosidase metabolism MeSH
Plant Roots microbiology MeSH
Plant Leaves microbiology MeSH
Molecular Typing MeSH
Plant Diseases microbiology MeSH
Siderophores metabolism MeSH
Solanum lycopersicum growth & development microbiology MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
beta-1,3-exoglucanase MeSH Browser
Chitinases MeSH
DNA, Bacterial MeSH
Glucan 1,3-beta-Glucosidase MeSH
Siderophores MeSH

Antagonistic Bacillus spp. displaying in vitro production of siderophore, chitinase, and β-1,3-glucanase were identified from dual culture assays. In independent greenhouse studies, seed bacterization and soil application of Bacillus atrophaeus S2BC-2 challenge inoculated with Fusarium oxysporum f.sp. lycopersici (FOL) and Alternaria solani (AS) recorded low percent disease index of 25.3 and 28.7, respectively, over nonbacterised pathogen control (44.3 and 56.4). The low disease incidence corroborated with tomato growth promotion with high vigor index (8,041.2) and fresh plant weight (82.5 g) on challenge inoculation with FOL. Analysis of root and leaf samples in rhizobacterial treatment challenged with FOL and AS revealed maximum induction of chitinase (1.9 and 1.7 U/mg of protein, respectively) and β-1,3-glucanase (23.5 and 19.2 U/mg of protein, respectively). In native gel activity assays, the rhizobacterial treatment on challenge inoculation strongly expressed three high intensity PO isoforms along with one low intensity isoform. In studies on genetic diversity of the Bacillus strains by repetitive extragenomic palindromic-polymerase chain reaction (REP-PCR) and amplified rDNA restriction analysis (ARDRA) patterns, ARDRA was more highly discriminant than REP-PCR and allowed grouping of the strains and differentiation of the antagonistic strains from other isolates.

Floriculture Pathology Laboratory Institute of Himalayan Bioresource Technology Council of Scientific and Industrial Research Palampur 176 061 Himachal Pradesh India

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