Screen-printed carbon electrodes modified by rhodium dioxide and glucose dehydrogenase
Status PubMed-not-MEDLINE Language English Country United States Media electronic
Document type Journal Article
PubMed
21528113
PubMed Central
PMC3083001
DOI
10.4061/2010/324184
Knihovny.cz E-resources
- Publication type
- Journal Article MeSH
The described glucose biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide, which functions as a mediator. The electrode is further modified by the enzyme glucose dehydrogenase, which is immobilized on the electrode's surface through electropolymerization with m-phenylenediamine. The enzyme biosensor was optimized and tested in model glucose samples. The biosensor showed a linear range of 500-5000 mg L(-1) of glucose with a detection limit of 210 mg L(-1) (established as 3σ) and response time of 39 s. When compared with similar glucose biosensors based on glucose oxidase, the main advantage is that neither ascorbic and uric acids nor paracetamol interfere measurements with this biosensor at selected potentials.
See more in PubMed
Nistor M, Csöregi E. Biosensors for food analysis. In: Grimes CA, Dickey CE, Pishko MV, editors. Encyclopedia of Sensors. Vol. 1. Stevenson Ranch, CA, USA: American Scientific Publishers; 2006. pp. 353–369.
Turner APF, Karube I, Wilson GS. Biosensors: Fundamentals and Applications. New York, NY, USA: Oxford University Press; 1987.
Moody GJ, Sangbera GS, Thomas JDR. Chemically immobilised bi-enzyme electrodes in the redox mediated mode for the low flow injection analysis of glucose and hypoxanthine. Analyst. 1987;112(1):65–70. PubMed
Okuma H, Takahashi H, Sekimukai S, Kawahara K, Akahoshi R. Mediated amperometric biosensor for hypoxanthine based on a hydroxymethylferrocene-modified carbon paste electrode. Analytica Chimica Acta. 1991;244(2):161–164.
Ricci F, Amine A, Moscone D, Palleschi G. A probe for NADH and HO amperometric detection at low applied potential for oxidase and dehydrogenase based biosensor applications. Biosensors and Bioelectronics. 2007;22(6):854–862. PubMed
Rodríguez MC, Rivas GA. An enzymatic glucose biosensor based on the codeposition of rhodium, iridium, and glucose oxidase onto a glassy carbon transducer. Analytical Letters. 2001;34(11):1829–1840.
Kotzian P, Brázdilová P, Kalcher K, Vytřas K. Determination of hydrogen peroxide, glucose and hypoxanthine using (bio)sensors based on ruthenium dioxide-modified screen-printed electrodes. Analytical Letters. 2005;38(7):1099–1113.
Razumiene J, Vilkanauskyte A, Gureviciene V, et al. New bioorganometallic ferrocene derivatives as efficient mediators for glucose and ethanol biosensors on PQQ-dependent dehydrogenases. Journal of Organometallic Chemistry. 2003;668(1-2):83–90.
Prieto-Simón B, Macanás J, Muñoz M, Fàbregas E. Evaluation of different mediator-modified screen-printed electrodes used in a flow system as amperometric sensors for NADH. Talanta. 2007;71(5):2102–2107. PubMed
Pappas ACH, Prodromidis MI, Karayannis MI. Flow monitoring of NADH consumption in bioassays based on packed-bed reactors bearing NAD-dependent dehydrogenases: determination of acetaldehyde using alcohol dehydrogenase. Analytica Chimica Acta. 2002;467(1-2):225–232.
Chen H-Y, Xu J-J. Amperometric enzyme biosensors. In: Grimes CA, Dickey CE, Pishko MV, editors. Encyclopedia of Sensors. Vol. 1. Stevenson Ranch, CA, USA: American Scientific Publishers; 2006. pp. 145–167.
Rothwell SA, McMahon CP, O’Neill RD. Effects of polymerization potential on the permselectivity of poly(o-phenylenediamine) coatings deposited on Pt-Ir electrodes for biosensor applications. Electrochimica Acta. 2010;55(3):1051–1060.
Sha Y, Gao Q, Qi B, Yang X. Electropolymerization of azure B on a screen-printed carbon electrode and its application to the determination of NADH in a flow injection analysis system. Microchimica Acta. 2004;148(3-4):335–341.
Li XG, Huang MR, Duan W, Yang YL. Novel multifunctional polymers from aromatic diamines by oxidative polymerizations. Chemical Reviews. 2002;102(9):2925–3030. PubMed
Kotzian P, Brázdilová P, Řezková S, Kalcher K, Vytřas K. Amperometric glucose biosensor based on rhodium dioxide-modified carbon ink. Electroanalysis. 2006;18(15):1499–1504.