We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cross-species amplification in Castor fiber was successful only in four loci, we tested also nine recently published C. canadensis loci in the Eurasian species. Eight of the published loci amplified; however, three were monomorphic. The number of alleles was lower in C. fiber than in C. canadensis at all loci tested.

School of Natural Resources University of Arizona Biosciences East Tucson AZ 85721 USA Wildlife Genetics Laboratory National Wildlife Research Center Wildlife Services USDA Fort Collins CO 80521 USA Department of Zoology Charles University Prague Faculty of Science Vinicna 7 CZ 128 44 Praha 2 Czech Republic USGS SBSC Sonoran Desert Research Station University of Arizona Tucson AZ 85721 USA

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Nuclear and mitochondrial genetic structure in the Eurasian beaver (Castor fiber) - implications for future reintroductions