Surface-enhanced laser desorption ionization/time-of-flight (SELDI-TOF) mass spectrometry (MS) as a phenotypic method for rapid identification of antibiotic resistance
Language English Country England, Great Britain Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
21624485
DOI
10.1016/j.anaerobe.2011.05.008
PII: S1075-9964(11)00093-X
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Bacterial * MeSH
- Escherichia coli chemistry MeSH
- Humans MeSH
- Microbial Sensitivity Tests methods MeSH
- Escherichia coli Proteins analysis MeSH
- Proteome analysis MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Escherichia coli Proteins MeSH
- Proteome MeSH
Based on experiments with 10 defined strains of Escherichia coli, we present a new method for bacterial phenotyping using SELDI-TOF mass spectrometry. Changes in bacterial protein profiles in the context of the time of cultivation and the antibiotic environment were minimal. Proteom subprofiling may further distinguish between strains with specific susceptibility to antimicrobials. Mass spec-based methods may become common in the future of bacterial pathogen identification in clinical microbiology diagnostics.
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