Analysis of ammonia-oxidizing bacteria dominating in lab-scale bioreactors with high ammonium bicarbonate loading
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Ammonia metabolism MeSH
- Bacterial Proteins genetics MeSH
- Bioreactors microbiology MeSH
- DNA, Bacterial chemistry genetics MeSH
- Nitrates metabolism MeSH
- Nitrites metabolism MeSH
- Phylogeny MeSH
- In Situ Hybridization, Fluorescence MeSH
- Bicarbonates metabolism MeSH
- Culture Media chemistry MeSH
- Molecular Sequence Data MeSH
- Nitrosomonas classification genetics metabolism MeSH
- Oxidation-Reduction MeSH
- Polymerase Chain Reaction MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Biota * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ammonium bicarbonate MeSH Browser
- Ammonia MeSH
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- Nitrates MeSH
- Nitrites MeSH
- Bicarbonates MeSH
- Culture Media MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
The ammonia-oxidizing bacterial community (AOB) was investigated in two types of laboratory-scale bioreactors performing partial oxidation of ammonia to nitrite or nitrate at high (80 mM) to extremely high (428 mM) concentrations of ammonium bicarbonate. At all conditions, the dominant AOB was affiliated to the Nitrosomonas europaea lineage as was determined by fluorescence in situ hybridization and polymerase chain reaction in combination with denaturing gradient gel electrophoresis. Molecular analysis of the mixed populations, based on the 16S rRNA and cbbL genes, demonstrated the presence of two different phylotypes of Nitrosomonas, while microbiological analysis produced a single phylotype, represented by three different morphotypes. One of the most striking features of the AOB populations encountered in the bioreactors was the domination of highly aggregated obligate microaerophilic Nitrosomonas, with unusual cellular and colony morphology, commonly observed in nitrifying bioreactors but rarely investigated by cultural methods. The latter is probably not an adaptation to stressful conditions created by high ammonia or nitrite concentrations, but oxygen seems to be a stressful factor in these bioreactors.
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GENBANK
GQ345128, GQ345129, GQ345130, GQ345131, JF508893, JF508894, JF508895, JF508896, JF508897, JF508898, JF508899, JF508900
