Prussian Blue acts as a mediator in a reagentless cytokinin biosensor
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
BB/F014651/1
Biotechnology and Biological Sciences Research Council - United Kingdom
PubMed
21801891
DOI
10.1016/j.aca.2011.06.018
PII: S0003-2670(11)00847-6
Knihovny.cz E-resources
- MeSH
- Arabidopsis enzymology MeSH
- Biosensing Techniques methods MeSH
- Cytokinins analysis MeSH
- Electrochemical Techniques methods MeSH
- Ferrocyanides chemistry MeSH
- Microelectrodes MeSH
- Oxidoreductases genetics metabolism MeSH
- Pichia metabolism MeSH
- Platinum chemistry MeSH
- Electroplating MeSH
- Recombinant Proteins genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- cytokinin oxidase MeSH Browser
- Cytokinins MeSH
- ferric ferrocyanide MeSH Browser
- Ferrocyanides MeSH
- Oxidoreductases MeSH
- Platinum MeSH
- Recombinant Proteins MeSH
An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N(6)-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface.
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