Phylogenetic diversity of bacterial communities in bovine rumen as affected by diets and microenvironments
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- bachor mikrobiologie MeSH
- Bacteria klasifikace genetika izolace a purifikace MeSH
- biodiverzita MeSH
- fylogeneze MeSH
- genomová knihovna MeSH
- krmivo pro zvířata mikrobiologie MeSH
- mikrobiální společenstva genetika MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- ribotypizace MeSH
- RNA ribozomální 16S analýza MeSH
- sekvenční analýza DNA MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- RNA ribozomální 16S MeSH
Phylogenetic analysis was conducted to examine ruminal bacteria in two ruminal fractions (adherent fraction vs. liquid fraction) collected from cattle fed with two different diets: forage alone vs. forage plus concentrate. One hundred forty-four 16S rRNA gene (rrs) sequences were obtained from clone libraries constructed from the four samples. These rrs sequences were assigned to 116 different operational taxonomic units (OTUs) defined at 0.03 phylogenetic distance. Most of these OTUs could not be assigned to any known genus. The phylum Firmicutes was represented by approximately 70% of all the sequences. By comparing to the OTUs already documented in the rumen, 52 new OTUs were identified. UniFrac, SONS, and denaturing gradient gel electrophoresis analyses revealed difference in diversity between the two fractions and between the two diets. This study showed that rrs sequences recovered from small clone libraries can still help identify novel species-level OTUs.
Zobrazit více v PubMed
Fed Proc. 1977 Feb;36(2):193-7 PubMed
Appl Environ Microbiol. 1988 May;54(5):1079-84 PubMed
Appl Environ Microbiol. 1980 Feb;39(2):376-81 PubMed
Biotechniques. 2004 May;36(5):808-12 PubMed
Trends Microbiol. 1997 Dec;5(12):483-8 PubMed
FEMS Microbiol Ecol. 2011 Apr;76(1):49-63 PubMed
Biosci Biotechnol Biochem. 2005 Mar;69(3):499-506 PubMed
Anaerobe. 2007 Apr;13(2):57-64 PubMed
Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1948-53 PubMed
Environ Microbiol. 2006 Apr;8(4):603-11 PubMed
ISME J. 2009 Dec;3(12):1365-73 PubMed
FEMS Microbiol Lett. 2011 Mar;316(1):51-60 PubMed
Appl Environ Microbiol. 2005 Dec;71(12):7724-36 PubMed
Appl Environ Microbiol. 2006 Oct;72(10):6773-9 PubMed
Appl Environ Microbiol. 2004 Aug;70(8):4800-6 PubMed
Environ Microbiol. 2005 Apr;7(4):530-43 PubMed
Appl Environ Microbiol. 2009 Dec;75(23):7537-41 PubMed
Appl Environ Microbiol. 2001 Apr;67(4):1565-74 PubMed
Microb Ecol. 2010 Apr;59(3):511-22 PubMed
Folia Microbiol (Praha). 2010 Jul;55(4):345-8 PubMed
Appl Environ Microbiol. 2005 Dec;71(12):8228-35 PubMed
Antonie Van Leeuwenhoek. 2004 Oct;86(3):263-81 PubMed
Reprod Nutr Dev. 2001 Mar-Apr;41(2):187-94 PubMed
