Diagnostic properties of three conventional selective plating media for selection of Bacillus cereus, B. thuringiensis and B. weihenstephanensis
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- Bacillus cereus klasifikace růst a vývoj izolace a purifikace metabolismus MeSH
- Bacillus thuringiensis klasifikace růst a vývoj izolace a purifikace metabolismus MeSH
- Bacillus klasifikace růst a vývoj izolace a purifikace metabolismus MeSH
- členovci mikrobiologie MeSH
- grampozitivní bakteriální infekce mikrobiologie MeSH
- kultivační média metabolismus MeSH
- lidé MeSH
- počet mikrobiálních kolonií metody MeSH
- potravinářská mikrobiologie MeSH
- půdní mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- kultivační média MeSH
The aim of this study was to assess the diagnostic properties of the two selective plating media and a chromogenic medium for identification of Bacillus cereus. The 324 isolates were B. cereus (37%), Bacillus weihenstephanensis (45%) or Bacillus thuringiensis (18%), as identified by a new combination of techniques. All isolates were growing on mannitol-egg yolk-polymyxin agar (MYP), and they did not form acid from mannitol. However, a significant lower number of B. thuringiensis isolates did not show lecithinase activity. All isolates were also growing on polymyxin-egg yolk-mannitol-bromothymol blue agar (PEMBA); however, 11% isolates indicated that they did produce acid from mannitol, and 15% isolates did not show any lecithinase activity. Five of the isolates did not grow at all on the chromogenic agar, and 14 of the growing isolates were β-glucosidase negative. It is concluded that the two recommended selective plating media MYP and PEMBA for detection of B. cereus group bacteria both have their limitations for identification of some B. cereus, B. weihenstephanensis or B. thuringiensis. However, MYP is preferable compared to PEMBA. The chromogenic medium has its own advantages and limitations, and some of the limitations seem to be solved by incubation at 30°C instead of the recommended 37°C.
Zobrazit více v PubMed
Syst Appl Microbiol. 2010 Jun;33(4):204-8 PubMed
FEMS Microbiol Rev. 2008 Jul;32(4):579-606 PubMed
Proteomics. 2005 Sep;5(14):3696-711 PubMed
FEMS Microbiol Lett. 2001 Aug 21;202(2):209-13 PubMed
J Appl Microbiol. 2001 Sep;91(3):421-6 PubMed
Clin Microbiol Rev. 2010 Apr;23(2):382-98 PubMed
J Med Microbiol. 1988 Mar;25(3):157-65 PubMed
Antonie Van Leeuwenhoek. 2006 Feb;89(2):239-49 PubMed
Mol Nutr Food Res. 2004 Dec;48(7):479-87 PubMed
Int J Food Microbiol. 2008 Jan 15;121(1):27-34 PubMed
Appl Environ Microbiol. 1987 Jun;53(6):1263-6 PubMed
