Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22085825
DOI
10.1016/j.cbpc.2011.10.009
PII: S1532-0456(11)00200-6
Knihovny.cz E-resources
- MeSH
- Antioxidants metabolism MeSH
- Diet MeSH
- Glutathione metabolism MeSH
- Glutathione Transferase metabolism MeSH
- Insect Hormones pharmacology MeSH
- Insect Proteins genetics metabolism MeSH
- Catalase genetics metabolism MeSH
- Pyrrolidonecarboxylic Acid analogs & derivatives pharmacology MeSH
- Larva drug effects genetics metabolism MeSH
- Manduca chemistry MeSH
- Oligopeptides pharmacology MeSH
- Oxidative Stress drug effects MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Gene Expression Regulation, Enzymologic drug effects MeSH
- Spodoptera drug effects genetics metabolism MeSH
- Superoxide Dismutase genetics metabolism MeSH
- Tannins administration & dosage MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- adipokinetic hormone MeSH Browser
- Antioxidants MeSH
- Glutathione MeSH
- Glutathione Transferase MeSH
- Insect Hormones MeSH
- Insect Proteins MeSH
- Catalase MeSH
- Pyrrolidonecarboxylic Acid MeSH
- Oligopeptides MeSH
- Superoxide Dismutase MeSH
- Tannins MeSH
The antioxidative potential of the Manduca sexta adipokinetic hormone (Manse-AKH) in the last instar larvae of Spodoptera littoralis (Noctuidae, Lepidoptera) was demonstrated after exposure to oxidative stress (OS) elicited by feeding on artificial diet containing tannic acid (TA). Determination of protein carbonyls (PCs) and reduced glutathione (GSH) levels, monitoring of activity of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferases (GSTs), as well as measuring of the mRNA expression of CAT and SOD were used as markers of the OS. Injection of the Manse-AKH (5 pmol per individual) reversed the OS status by mitigation of PCs formation and by stimulation of glutathione-S-transferases (GSTs) activity. The CAT and SOD mRNA expression was significantly suppressed after the Manse-AKH injection while activity of these enzymes was not affected. These results indicate that diminishing of OS after the AKH injection might be a result of activation of specific enzymatic pathway possibly at the post-translational level rather than a direct effect on regulation of antioxidant marker genes at the transcriptional level.
References provided by Crossref.org
Elimination of certain honeybee venom activities by adipokinetic hormone
Hormonal Regulation of Response to Oxidative Stress in Insects-An Update
