Phenotypic characterization and RAPD fingerprinting of Vibrio parahaemolyticus and Vibrio alginolyticus isolated during Tunisian fish farm outbreaks
Language English Country United States Media print-electronic
Document type Journal Article
- MeSH
- DNA Fingerprinting MeSH
- Disease Outbreaks veterinary MeSH
- Phenotype MeSH
- Vibrio Infections epidemiology microbiology veterinary MeSH
- Sea Bream microbiology MeSH
- Fish Diseases epidemiology microbiology MeSH
- Bass microbiology MeSH
- Random Amplified Polymorphic DNA Technique methods MeSH
- Vibrio alginolyticus genetics isolation & purification MeSH
- Vibrio parahaemolyticus genetics isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Tunisia MeSH
The genus Vibrio is characterized by a large number of species and some of them are human pathogens causing gastrointestinal and wound infections through the ingestion or manipulation of contaminated fishes and shellfish including Vibrio parahaemolyticus and Vibrio alginolyticus. In this study, we reported the phenotypic and molecular characterization of 9 V. parahaemolyticus and 27 V. alginolyticus strains isolated from outbreaks affecting cultured Gilthead sea bream (Sparus aurata L.) and Sea bass (Dicentrarchus labrax) along the Tunisian coast from 2008 to 2009. All isolates were tested for the presence of DNase, caseinase, protease, lipase, amylase, gelatinase, hemolytic activity and antibacterial resistance to different drugs. Randomly amplified polymorphic DNA was used to examine the genetic relatedness among the V. parahaemolyticus and V. alginolyticus strains.
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