Uptake and incorporation of sialic acid by the tick Ixodes ricinus
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
GM24349
NIGMS NIH HHS - United States
PubMed
22781367
DOI
10.1016/j.jinsphys.2012.06.016
PII: S0022-1910(12)00163-1
Knihovny.cz E-resources
- MeSH
- Epitopes MeSH
- Gastrointestinal Tract metabolism MeSH
- Immunohistochemistry MeSH
- Ixodes metabolism MeSH
- N-Acetylneuraminic Acid metabolism MeSH
- Malpighian Tubules metabolism MeSH
- Polysaccharides metabolism MeSH
- Salivary Glands metabolism MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Microscopy, Electron, Transmission MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Names of Substances
- Epitopes MeSH
- N-Acetylneuraminic Acid MeSH
- Polysaccharides MeSH
We describe the detection of sialylated N-linked glycans in partially fed Ixodes ricinus tick females using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Sialylated glycans were detected in salivary glands as well as in tick guts and we propose the host origin of these structures. In addition, we mapped the transport of sialylated structures from the blood meal through the gut to the salivary glands using electron microscopy. Specific localization of sialylated glycans to basement membranes of salivary glands was observed. Finally, the influence of the sample preparation methods for electron microscopy on ultrastructure and immunogold labeling was evaluated.
References provided by Crossref.org
A bite so sweet: the glycobiology interface of tick-host-pathogen interactions
