Collagenolytic potential of rat liver myofibroblasts
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23173684
DOI
10.33549/physiolres.932399
PII: 932399
Knihovny.cz E-resources
- MeSH
- Actins metabolism MeSH
- Biomarkers metabolism MeSH
- Time Factors MeSH
- Cytokines metabolism MeSH
- Cytoskeleton enzymology MeSH
- Fibrin metabolism MeSH
- Immunohistochemistry MeSH
- Liver cytology enzymology MeSH
- Collagen Type I metabolism MeSH
- Collagenases genetics metabolism MeSH
- Rats MeSH
- Cells, Cultured MeSH
- RNA, Messenger metabolism MeSH
- Myofibroblasts enzymology MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Rats, Sprague-Dawley MeSH
- Cell Separation methods MeSH
- Cell Shape MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Biomarkers MeSH
- Cytokines MeSH
- Fibrin MeSH
- Collagen Type I MeSH
- Collagenases MeSH
- RNA, Messenger MeSH
- smooth muscle actin, rat MeSH Browser
Rat liver myofibroblasts (MFB) were isolated by repeated passaging of nonparenchymal liver cell fraction. They were cultured on polystyrene Petri dishes, on fibrin or on type I collagen gels for 5 days. Quantitative RT-PCR, Western blotting, zymography and immunocytochemistry were used to study differences in cell morphology and protein expression. MFB were large and spread on plastic substrate, with prominent alpha-smooth muscle (alpha-SMA) fibres. They turned much smaller and elongated on collagen which was accompanied by the rearrangement of the cytoskeleton and a decrease in alpha-SMA and beta-actin content. Collagen gel induced the expression of a group of metalloproteinases (MMP-2, -3, -9, -13), on mRNA and protein level which resulted in the degradation of the gel. This response was accompanied by changes in the mRNA expression of cytokines of TGF-beta family, CTGF and interleukin-6, as well as of osteopontin and thrombospondin-2 that are involved in metalloproteinases (MMPs) regulation. The expression of MMPs substrates, collagen types I, IV and XII did not change or decreased. The effects of fibrin gels on MFB were milder than those of collagen. MFB assumed to deposit collagen and other ECM components in fibrotic liver, besides hepatic stellate cells, also possess a great collagenolytic potential.
References provided by Crossref.org
Collagen matrix as a tool in studying fibroblastic cell behavior
