PEG-modified macroporous poly(glycidyl methacrylate) and poly(2-hydroxyethyl methacrylate) microspheres to reduce non-specific protein adsorption
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adsorption MeSH
- Biotechnology methods MeSH
- Chymotrypsin chemistry MeSH
- gamma-Globulins chemistry MeSH
- Polymethacrylic Acids chemistry MeSH
- Microspheres * MeSH
- Molecular Structure MeSH
- Polyethylene Glycols chemistry MeSH
- Polyhydroxyethyl Methacrylate chemistry MeSH
- Iodine Radioisotopes chemistry MeSH
- Serum Albumin, Bovine chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chymotrypsin MeSH
- gamma-Globulins MeSH
- Polymethacrylic Acids MeSH
- Polyethylene Glycols MeSH
- polyglycidyl methacrylate MeSH Browser
- Polyhydroxyethyl Methacrylate MeSH
- Iodine Radioisotopes MeSH
- Serum Albumin, Bovine MeSH
To minimize non-specific protein adsorption on macroporous poly(glycidyl methacrylate) and poly(2-hydroxyethyl methacrylate) microspheres containing amino and/or carboxyl groups, the microspheres are coated with α,ω-bis-carboxy poly(ethylene glycol) and amino-terminated poly(ethylene glycol-co-propylene glycol) or α-methoxy-ω-amino poly(ethylene glycol). Adsorption of bovine serum albumin (BSA), γ-globulin, (125) I-BSA, pepsin, and chymotrypsin on neat and PEGylated microspheres is determined by UV-VIS spectroscopy of supernatants and eluates or by measurement of radioactivity in an ionization chamber. Neat and PEGylated microspheres adsorb 0.8-70% and 0.02-44% of protein, respectively.
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