Integrated chip electrophoresis and magnetic particle isolation used for detection of hepatitis B virus oligonucleotides
Language English Country Germany Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- DNA, Viral isolation & purification MeSH
- Electrophoresis, Microchip instrumentation MeSH
- Hepatitis B diagnosis MeSH
- Humans MeSH
- Limit of Detection MeSH
- Magnetite Nanoparticles chemistry MeSH
- Oligonucleotides isolation & purification MeSH
- Hepatitis B virus isolation & purification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Viral MeSH
- Magnetite Nanoparticles MeSH
- Oligonucleotides MeSH
Rapid and sensitive detection is a key step in the effective and early response to the global hazard of various viral diseases. In this study, an integrated isolation of hepatitis B virus (HBV)-specific DNA fragment by magnetic nanoparticles (MNPs) and its immediate analysis by microchip CGE was performed. Microfluidic CE chip was used to accommodate the complete process of viral DNA isolation by MNPs including hybridization and thermal denaturation followed by CE separation. Beforehand, calibration curves of HBV fragments were constructed. For isolation by MNPs, specific streptavidin-biotin interaction was used to bind complementary HBV fragment to magnetic particles. After analysis of isolated HBV by regular MNPs method, innovative approach was performed. The commercial CE chip (Bio-rad) was successfully used to execute HBV fragment isolation. Detection using LIF with detection limit of 1 ng/mL was accomplished.
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