Serotonin modulates outward potassium currents in mouse olfactory receptor neurons
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23590600
DOI
10.33549/physiolres.932413
PII: 932413
Knihovny.cz E-resources
- MeSH
- Serotonin 5-HT1 Receptor Antagonists pharmacology MeSH
- Olfactory Receptor Neurons drug effects metabolism MeSH
- Potassium Channels drug effects metabolism MeSH
- Membrane Potentials MeSH
- RNA, Messenger metabolism MeSH
- Mice MeSH
- Receptor, Serotonin, 5-HT1A drug effects genetics metabolism MeSH
- Receptor, Serotonin, 5-HT1B drug effects genetics metabolism MeSH
- Serotonin metabolism MeSH
- Signal Transduction MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Serotonin 5-HT1 Receptor Antagonists MeSH
- Potassium Channels MeSH
- RNA, Messenger MeSH
- Receptor, Serotonin, 5-HT1A MeSH
- Receptor, Serotonin, 5-HT1B MeSH
- Serotonin MeSH
Monoaminergic neurotransmitter 5-hydroxytryptamine (5-HT), also known as serotonin, plays important roles in modulating the function of the olfactory system. However, thus far, the knowledge about 5-HT and its receptors in olfactory receptor neurons (ORNs) and their physiological role have not been fully characterized. In the present study, reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed the presence of 5-HT(1A) and 5-HT(1B) receptor subtypes in mouse olfactory epithelium at the mRNA level. With subtype selective antibodies and standard immunohistochemical techniques, both receptor subtypes were found to be positively labeled. To further elucidate the molecular mechanisms of 5-HT act on the peripheral olfactory transduction, the whole-cell patch clamp techniques were used on freshly isolated ORNs. We found that 5-HT decreased the magnitude of outward K(+) current in a dose-dependent manner and these inhibitory effects were markedly attenuated by the 5-HT(1A) receptor blocker WAY-100635 and the 5-HT(1B) receptor antagonist GR55562. These data suggested that 5-HT may play a role in the modulation of peripheral olfactory signals by regulating outward potassium currents, both 5-HT(1A) and 5-HT(1B) receptors were involved in this regulation.
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