Expression analysis of MND1/GAJ, SPATA22, GAPDHS and ACR genes in testicular biopsies from non-obstructive azoospermia (NOA) patients
Language English Country England, Great Britain Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23675907
PubMed Central
PMC3664614
DOI
10.1186/1477-7827-11-42
PII: 1477-7827-11-42
Knihovny.cz E-resources
- MeSH
- Acrosin genetics MeSH
- Azoospermia genetics pathology MeSH
- Biopsy MeSH
- Adult MeSH
- Fertilization MeSH
- Glyceraldehyde-3-Phosphate Dehydrogenases genetics MeSH
- Sperm Injections, Intracytoplasmic MeSH
- Middle Aged MeSH
- Humans MeSH
- Testicular Diseases genetics pathology MeSH
- Sperm Retrieval MeSH
- Oligospermia genetics pathology MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Cell Cycle Proteins genetics MeSH
- Sertoli Cell-Only Syndrome genetics pathology MeSH
- Spermatogenesis genetics MeSH
- Gene Expression Profiling MeSH
- Pregnancy MeSH
- Testis metabolism pathology MeSH
- Pregnancy Rate MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acrosin MeSH
- Glyceraldehyde-3-Phosphate Dehydrogenases MeSH
- MND1 protein, human MeSH Browser
- Cell Cycle Proteins MeSH
- SPATA22 protein, human MeSH Browser
BACKGROUND: High-throughput studies provide a wide spectrum of genes for use as predictive markers during testicular sperm extraction (TESE) in combination with ICSI. In this work, we used the specimens from testicular biopsies of men with non-obstructive azoospermia who underwent TESE to investigate the expression of spermatogenesis-related genes MND1, SPATA22, GAPDHS and ACR. METHODS: Testicular biopsy specimens were subdivided into three groups: hypospermatogenesis (HS); maturation arrest (MA); and Sertoli cell-only syndrome (SCO). The levels of expression of the spermatogenesis-related genes MND1, SPATA22, GAPDHS and ACR in the testes were compared among these three groups using the reverse transcription polymerase chain reaction (RT-PCR) technique. RESULTS: Analysis of the expression of spermatogenic genes in human testes with abnormal spermatogenesis showed different expression patterns in patients from different groups. Fertilization rate for studied set of patients was 66% and pregnancy rate 29%. For HS group fertilization rate was 72% and pregnancy rate 32%, while for MA group fertilization and pregnancy rates were 54% and 26%, respectively. Fertilization rates in relation to the studied genes were uniformly around 70%, pregnancy rates for ACR and GAPDHS genes were surprisingly low at 6% and 8% correspondingly. CONCLUSIONS: Analysis of the expression of genes involved in spermatogenesis can be a fast additional test for the level of spermatogenesis in testicular samples.
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