Real time monitoring of population dynamics in concurrent bacterial growth using SIFT-MS quantification of volatile metabolites
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23801325
DOI
10.1039/c3an00472d
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Escherichia coli growth & development metabolism MeSH
- Mass Spectrometry methods MeSH
- Serratia marcescens growth & development metabolism MeSH
- Volatile Organic Compounds chemistry metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Volatile Organic Compounds MeSH
Population dynamics of three different bacterial species, Serratia rubidaea (R), Serratia marcescens (F) and Escherichia coli (Ec), growing in single or mixed populations in liquid media, was monitored by real time headspace quantification of volatile compounds using selected ion flow tube mass spectrometry, SIFT-MS. The three bacterial species interact with each other in a competitive fashion in a way similar to the game "rock-paper-scissors" (R-Ec-F). The concentrations of volatile metabolites (ammonia, ethanol, acetaldehyde, propanol, acetoin, acetone and acetic acid) were measured in the headspace of the individual species and of their mixtures continuously for 24 hour periods. The results demonstrate that dynamics in bacterial cultures can be monitored using SIFT-MS in real time.
References provided by Crossref.org
Variations and heredity in bacterial colonies
