Basic nuclear processes affected by histone acetyltransferases and histone deacetylase inhibitors
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23895652
DOI
10.2217/epi.13.38
Knihovny.cz E-zdroje
- MeSH
- acetylace účinky léků MeSH
- apoptóza účinky léků MeSH
- buněčné jádro účinky léků metabolismus MeSH
- buňky K562 MeSH
- DNA vazebné proteiny MeSH
- DNA-helikasy MeSH
- epigeneze genetická MeSH
- histonacetyltransferasy genetika metabolismus MeSH
- histony metabolismus MeSH
- inhibitory histondeacetylas farmakologie MeSH
- jaderné proteiny genetika MeSH
- kyseliny hydroxamové farmakologie MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádorový supresorový protein p53 genetika MeSH
- NF-kappa B genetika MeSH
- oprava DNA účinky léků MeSH
- protein BRCA2 genetika MeSH
- regulace genové exprese MeSH
- rekombinasa Rad51 genetika MeSH
- RNA genetika MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- signální transdukce účinky léků MeSH
- spliceozomy genetika metabolismus MeSH
- stanovení celkové genové exprese MeSH
- transkripční faktory genetika metabolismus MeSH
- vorinostat MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- BRCA2 protein, human MeSH Prohlížeč
- DNA vazebné proteiny MeSH
- DNA-helikasy MeSH
- histonacetyltransferasy MeSH
- histony MeSH
- inhibitory histondeacetylas MeSH
- jaderné proteiny MeSH
- kyseliny hydroxamové MeSH
- nádorový supresorový protein p53 MeSH
- NF-kappa B MeSH
- protein BRCA2 MeSH
- RAD51 protein, human MeSH Prohlížeč
- RAD54L protein, human MeSH Prohlížeč
- rekombinasa Rad51 MeSH
- RNA MeSH
- transkripční faktory MeSH
- vorinostat MeSH
AIM: The optimal balance between histone acetylation and deacetylation is important for proper gene function. Therefore, we addressed how inhibitors of histone-modifying enzymes can modulate nuclear events, including replication, transcription, splicing and DNA repair. MATERIALS & METHODS: Changes in cell signaling pathways upon treatment with histone acetyltransferases and/or histone deacetylase inhibitors were studied by cDNA microarrays and western blots. RESULTS: We analyzed the effects of the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) and the histone acetylase inhibitor MG149. SAHA altered the expression of factors involved in DNA replication complexes, basal transcription and the spliceosome pathway. DNA repair-related genes, including Rad51, Rad54 and BRCA2, were significantly downregulated by SAHA. However, MG149 had no effect on the investigated nuclear processes, with the exception of the spliceosome network and Sestrins, involved in DNA repair. CONCLUSION: Based on our results, we propose that the studied epigenetic drugs have the distinct potential to affect specific cell signaling pathways depending on their respective molecular targets.
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