Relative prevalence of Mycobacterium marinum in fish collected from aquaria and natural freshwaters in central Europe
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23952681
DOI
10.1111/jfd.12135
Knihovny.cz E-resources
- Keywords
- ecology, fish mycobacteriosis, molecular diagnostics,
- MeSH
- Mycobacterium Infections, Nontuberculous epidemiology microbiology veterinary MeSH
- Bacterial Proteins genetics MeSH
- Muscle, Skeletal microbiology MeSH
- Molecular Sequence Data MeSH
- Multiplex Polymerase Chain Reaction veterinary MeSH
- Mycobacterium marinum genetics isolation & purification MeSH
- Fish Diseases epidemiology microbiology MeSH
- Colony Count, Microbial veterinary MeSH
- Prevalence MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Fishes MeSH
- Sequence Analysis, DNA veterinary MeSH
- Fresh Water microbiology MeSH
- Intestines microbiology MeSH
- Gills microbiology MeSH
- Animals, Zoo microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Europe MeSH
- Names of Substances
- Bacterial Proteins MeSH
- RNA, Ribosomal, 16S MeSH
A survey was carried out on occurrence of Mycobacterium marinum in fish kept in aquaria and those living in their natural environment. Species-specific qPCR targeting the erp and IS2404 genes together with the conventional culture method were used. The analysis of 72 ornamental fish (n = 216 samples: gills, muscle and intestine) collected from aquaria revealed the presence of M. marinum in 30 individuals (41.7%) of whom 17 (23.6%) were later culture positive. Culture-independent detection revealed the presence of M. marinum in 16 of 83 environmental samples (19.3%) collected in aquaria. The presence of viable M. marinum cells was later confirmed in 5 samples (6.0%). No qPCR or culture positivity was observed when 123 groundwater fish and their corresponding environmental samples (n = 142) were analysed.
References provided by Crossref.org
Fish tank granuloma caused by Mycobacterium marinum in two aquarists: two case reports
