Chemoenzymatic synthesis of α-L-rhamnosides using recombinant α-L-rhamnosidase from Aspergillus terreus
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24012095
DOI
10.1016/j.biortech.2013.08.083
PII: S0960-8524(13)01313-8
Knihovny.cz E-resources
- Keywords
- Aspergillus terreus, Glycosylation, Phenolic hydroxyl, Reverse hydrolysis, α-l-Rhamnosidase,
- MeSH
- Aspergillus genetics metabolism MeSH
- Glycoside Hydrolases biosynthesis MeSH
- Recombination, Genetic * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- alpha-L-rhamnosidase MeSH Browser
- Glycoside Hydrolases MeSH
This study describes an efficient, large scale fermentation of a recombinant α-L-rhamnosidase originating from Aspergillus terreus. High-cell-density Pichia pastoris fermentation resulted in yields up to 627 U/L/h. The recombinant enzyme was used for the reverse rhamnosylation of various small organic compounds. A full factorial experimental design setup was applied to identify the importance of temperature, substrate concentrations, solvent type and concentration as well as the acidity of the reaction mixture. Careful optimization of these parameters allowed the synthesis of a range of α-L-rhamnosides among which cyclohexyl α-L-rhamnopyranoside, anisyl α-L-rhamnopyranoside and 2-phenylethyl α-L-rhamnopyranoside. In addition, α-L-rhamnosylation of phenolic hydroxyls in phenols such as hydroquinone, resorcinol, catechol and phenol was observed, which is a rather unique reaction catalyzed by glycosidases.
References provided by Crossref.org
"Sweet Flavonoids": Glycosidase-Catalyzed Modifications
