Chemoenzymatic synthesis of α-L-rhamnosides using recombinant α-L-rhamnosidase from Aspergillus terreus
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24012095
DOI
10.1016/j.biortech.2013.08.083
PII: S0960-8524(13)01313-8
Knihovny.cz E-zdroje
- Klíčová slova
- Aspergillus terreus, Glycosylation, Phenolic hydroxyl, Reverse hydrolysis, α-l-Rhamnosidase,
- MeSH
- Aspergillus genetika metabolismus MeSH
- glykosidhydrolasy biosyntéza MeSH
- rekombinace genetická * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alpha-L-rhamnosidase MeSH Prohlížeč
- glykosidhydrolasy MeSH
This study describes an efficient, large scale fermentation of a recombinant α-L-rhamnosidase originating from Aspergillus terreus. High-cell-density Pichia pastoris fermentation resulted in yields up to 627 U/L/h. The recombinant enzyme was used for the reverse rhamnosylation of various small organic compounds. A full factorial experimental design setup was applied to identify the importance of temperature, substrate concentrations, solvent type and concentration as well as the acidity of the reaction mixture. Careful optimization of these parameters allowed the synthesis of a range of α-L-rhamnosides among which cyclohexyl α-L-rhamnopyranoside, anisyl α-L-rhamnopyranoside and 2-phenylethyl α-L-rhamnopyranoside. In addition, α-L-rhamnosylation of phenolic hydroxyls in phenols such as hydroquinone, resorcinol, catechol and phenol was observed, which is a rather unique reaction catalyzed by glycosidases.
Citace poskytuje Crossref.org
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