Lichen secondary metabolites as DNA-interacting agents
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24269500
DOI
10.1016/j.tiv.2013.11.003
PII: S0887-2333(13)00307-X
Knihovny.cz E-resources
- Keywords
- DNA-binding, Lichens, Topoisomerase I, II,
- MeSH
- Benzoates pharmacology MeSH
- Benzofurans pharmacology MeSH
- Circular Dichroism MeSH
- DNA drug effects metabolism MeSH
- Emodin analogs & derivatives pharmacology MeSH
- Hydroxybenzoates pharmacology MeSH
- Topoisomerase I Inhibitors pharmacology MeSH
- Topoisomerase II Inhibitors pharmacology MeSH
- Intercalating Agents pharmacology MeSH
- Kinetics MeSH
- Nucleic Acid Conformation MeSH
- Humans MeSH
- Lichens chemistry metabolism MeSH
- Cattle MeSH
- Spectrophotometry, Ultraviolet MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- atranorin MeSH Browser
- Benzoates MeSH
- Benzofurans MeSH
- calf thymus DNA MeSH Browser
- DNA MeSH
- Emodin MeSH
- gyrophoric acid MeSH Browser
- Hydroxybenzoates MeSH
- Topoisomerase I Inhibitors MeSH
- Topoisomerase II Inhibitors MeSH
- Intercalating Agents MeSH
- physcione MeSH Browser
- usnic acid MeSH Browser
A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3×10(5) to 2.4×10(7)M(-1) and the percentage of hypochromism was found to be 16-34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25μM).
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