Lichen secondary metabolites as DNA-interacting agents
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24269500
DOI
10.1016/j.tiv.2013.11.003
PII: S0887-2333(13)00307-X
Knihovny.cz E-zdroje
- Klíčová slova
- DNA-binding, Lichens, Topoisomerase I, II,
- MeSH
- benzoáty farmakologie MeSH
- benzofurany farmakologie MeSH
- cirkulární dichroismus MeSH
- DNA účinky léků metabolismus MeSH
- emodin analogy a deriváty farmakologie MeSH
- hydroxybenzoáty farmakologie MeSH
- inhibitory topoisomerasy I farmakologie MeSH
- inhibitory topoisomerasy II farmakologie MeSH
- interkalátory farmakologie MeSH
- kinetika MeSH
- konformace nukleové kyseliny MeSH
- lidé MeSH
- lišejníky chemie metabolismus MeSH
- skot MeSH
- spektrofotometrie ultrafialová MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- atranorin MeSH Prohlížeč
- benzoáty MeSH
- benzofurany MeSH
- calf thymus DNA MeSH Prohlížeč
- DNA MeSH
- emodin MeSH
- gyrophoric acid MeSH Prohlížeč
- hydroxybenzoáty MeSH
- inhibitory topoisomerasy I MeSH
- inhibitory topoisomerasy II MeSH
- interkalátory MeSH
- physcione MeSH Prohlížeč
- usnic acid MeSH Prohlížeč
A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3×10(5) to 2.4×10(7)M(-1) and the percentage of hypochromism was found to be 16-34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25μM).
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