Determination of auxin transport parameters on the cellular level
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Metabolic Flux Analysis MeSH
- Arabidopsis cytology metabolism MeSH
- Biological Transport MeSH
- Cell Membrane metabolism MeSH
- Cell Culture Techniques MeSH
- Kinetics MeSH
- Cells, Cultured MeSH
- Indoleacetic Acids metabolism MeSH
- Arabidopsis Proteins metabolism MeSH
- Nicotiana cytology metabolism MeSH
- Carrier Proteins metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Indoleacetic Acids MeSH
- Arabidopsis Proteins MeSH
- Carrier Proteins MeSH
The accumulation of radioactively labelled compounds in cells is frequently used for the determination of activities of various transport systems located at the plasma membrane, including the system for carrier-mediated transport of plant hormone auxin. The measurements of auxin transport could be performed on the tissue level as well, but for more precise quantitative analysis of activity of individual auxin carriers the model of plant cell cultures represents an invaluable tool. Here, we describe the method for the determination of the activities of auxin influx and efflux carriers in plant cells grown in a suspension using radiolabelled synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA). By making use of specific inhibitors of active auxin influx and efflux, as well as cell lines overexpressing or silencing particular auxin carriers, this method allows the determination of kinetic parameters of auxin flow across the plasma membrane and the activity of those carriers.
References provided by Crossref.org
What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo
