The antimicrobial action of histones in the reproductive tract of cow
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24361880
DOI
10.1016/j.bbrc.2013.12.077
PII: S0006-291X(13)02148-7
Knihovny.cz E-zdroje
- Klíčová slova
- Antimicrobial properties, Bovine reproductive tract, Extracellular histones, Histone-like proteins,
- MeSH
- antiinfekční látky metabolismus MeSH
- dialýza MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- histony chemie metabolismus MeSH
- hmotnostní spektrometrie MeSH
- skot MeSH
- tělesné tekutiny metabolismus MeSH
- vejcovody u zvířat metabolismus MeSH
- ženské pohlavní orgány metabolismus MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antiinfekční látky MeSH
- histony MeSH
An infection of any part of female reproductive tract can severely interfere with fertility and reproduction. The fluids and epithelium from the lumen of the female reproductive tract (uterus, oviduct and ovarian follicle) are a known source of antimicrobial action in several species. In this study, we compared the antimicrobial properties of fluids from the reproductive tract of a cow. After removal of small molecules, we demonstrated that there is an antimicrobial activity connected with a fraction of compounds with a molecular mass range between 3500 and 30,000. The most probable candidates responsible for the observed antimicrobial effect were subsequently identified by mass spectroscopy as histones H2A type 2-C, H2B type 1-K, H3.3, and H4. The antimicrobial role of histone H2B was further confirmed by using an antibody against this histone.
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