Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24388242
DOI
10.1016/j.chroma.2013.12.039
PII: S0021-9673(13)01920-1
Knihovny.cz E-zdroje
- Klíčová slova
- Body fluid samples, Capillary electrophoresis, Contactless conductivity detection, Ethylene glycol intoxication, Methanol intoxication, Toxic metabolites,
- MeSH
- elektroforéza kapilární metody MeSH
- elektrolyty MeSH
- formiáty izolace a purifikace MeSH
- glykoláty izolace a purifikace MeSH
- lidé MeSH
- tělesné tekutiny chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- elektrolyty MeSH
- formiáty MeSH
- formic acid MeSH Prohlížeč
- glycolic acid MeSH Prohlížeč
- glykoláty MeSH
A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6min in a fused silica capillary of 25μm I.D. using an electrolyte comprising 50mM l-histidine and 50mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3μM and limits of quantitation 1.3, 1.9 and 4.2μM for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice.
Bioanalytical Instrumentation CEITEC Masaryk University Veveří 97 60200 Brno Czech Republic
Institute of Chemistry Masaryk University Kotlářská 2 61137 Brno Czech Republic
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