The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.

Institute of Chemical Process Fundamentals of the ASCR v v i Rozvojová 2 135 16502 Prague 6 Czech Republic

Institute of Experimental Botany of the ASCR v v i Isotope Laboratory Vídeňská 1083 14220 Prague 4 Czech Republic ; Institute of Chemical Technology Prague Faculty of Food and Biochemical Technology Department of Chemistry of Natural Compounds Technická 5 16628 Prague 6 Czech Republic

Institute of Organic Chemistry and Biochemistry of the ASCR v v i Flemingovo Náměstí 2 16610 Prague 6 Czech Republic

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