Skin wound healing in obese and lean male adolescent rats submitted to pre-weaning litter size manipulation
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24594053
DOI
10.14712/fb2014060010021
PII: file/5999/fb2014a0003.pdf
Knihovny.cz E-resources
- MeSH
- Wound Healing * MeSH
- Homeostasis MeSH
- Insulin metabolism MeSH
- Rats MeSH
- Skin pathology MeSH
- Metabolic Syndrome genetics MeSH
- Disease Models, Animal MeSH
- Animals, Newborn MeSH
- Obesity genetics MeSH
- Weaning MeSH
- Tensile Strength MeSH
- Rats, Sprague-Dawley MeSH
- Body Weight MeSH
- Animal Nutrition Sciences MeSH
- Litter Size MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Insulin MeSH
We investigated whether early postnatal over-nutrition affects normal course of skin wound healing. To induce over-nutrition the litter size was adjusted on the first day after birth to four pups/nest (small litters). In parallel, as a control, normal nests of 10 pups/nest (normal litters) were used. For the wound healing experiment 30 male Sprague-Dawley rats, 15 from normal nests and 15 from small nests, were used. Two parallel full-thickness skin incisions and two full-thickness excisions were performed on the back of each rat. Samples for histological examination (excisions) and wound tensile strength measurement (incisions) were collected on days 2, 6, and 14 after surgery. Our study demonstrates that rats from the small nests had enhanced plasma levels of insulin and enhanced body weight/fat parameters. Furthermore, in small nests, rats that expressed the above-mentioned symptoms displayed slight improvement of epidermis regeneration, accelerated demarcation line formation, and increased wound tensile strength. From this point of view the small nest model used in the present experiment is helpful for exploration whether these acquired changes might be considered as a sufficient essential factor involved in the regulation of metabolic homeostasis and wound repair in juvenile obese male rats. Nevertheless, further studies need to be performed to verify the present findings also on other animal models and humans and to describe the exact underlying mechanism.
Department of Forensic Medicine Pavol Jozef Šafárik University Košice Slovak Republic
Department of Pathological Physiology Pavol Jozef Šafárik University Košice Slovak Republic
Institute of Anatomy 1st Faculty of Medicine Charles University Prague Czech Republic
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