Electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Bidirectional isotachophoresis, Electrofocusing, Electrolyte system, Moving boundary electrophoresis, Wide pH range,
- MeSH
- Coloring Agents chemistry isolation & purification MeSH
- Electrolytes chemistry MeSH
- Isoelectric Focusing methods MeSH
- Isotachophoresis methods MeSH
- Hydrogen-Ion Concentration MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Coloring Agents MeSH
- Electrolytes MeSH
In this paper, we suggest new electrolyte system for fast preparative electrofocusing in wide pH range. It is based on bidirectional ITP with multiple counterions and spacers created by commercially available defined simple buffers. The migration course of proposed focusing model can be simulated in advance by using separation conditions and electrolyte components that are consequently applied during the experiments. The suggested electrolyte system allows high current densities at the initial stages of focusing without danger of local overheating, which strongly reduces the time needed for analysis completion. The performance of the electrolyte system is demonstrated by the focusing of synthetic colored low molecular weight indicators and proteins in the arrangements with both linear narrow strip and nonwoven fabric sheet with continuous flow.
References provided by Crossref.org
