Multispecies biofilm in an artificial wound bed--A novel model for in vitro assessment of solid antimicrobial dressings
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24880129
DOI
10.1016/j.mimet.2014.05.008
PII: S0167-7012(14)00130-4
Knihovny.cz E-resources
- Keywords
- Anti-biofilm substances, Artificial wound bed, In vitro testing, Multi-species wound biofilm model,
- MeSH
- Anti-Infective Agents administration & dosage MeSH
- Bacteria classification drug effects genetics growth & development MeSH
- Genes, Bacterial MeSH
- Bacterial Load MeSH
- Biofilms drug effects MeSH
- Chronic Disease MeSH
- Gene Expression MeSH
- Phenotype MeSH
- Wound Infection drug therapy microbiology MeSH
- Iodine administration & dosage MeSH
- Bandages * MeSH
- In Vitro Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Infective Agents MeSH
- Iodine MeSH
Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48h post-transplant. The applicability of the model was evaluated by testing two common iodine wound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant.
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