Motility and fertilization ability of sterlet Acipenser ruthenus testicular sperm after cryopreservation
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25058859
DOI
10.1016/j.cryobiol.2014.07.008
PII: S0011-2240(14)00196-5
Knihovny.cz E-resources
- Keywords
- Sperm maturation, Sterlet, Sturgeon conservation,
- MeSH
- Fertilization in Vitro MeSH
- Cryopreservation methods veterinary MeSH
- Sperm Motility MeSH
- Fishes physiology MeSH
- Spermatozoa cytology MeSH
- Testis cytology MeSH
- Semen Preservation methods veterinary MeSH
- Wolffian Ducts cytology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Sturgeon spermatozoa are immotile in the testis and acquire the potential for motility after contact with urine in Wolffian duct. The present study tested if in vitro incubation of testicular sperm in seminal fluid from Wolffian duct sperm leads to the acquisition of sperm fertilization ability. Sterlet sperm was taken from the testes, matured in vitro and cryopreserved. The fertility and motility of cryopreserved semen were tested. Matured testicular sperm showed freeze-thaw survival rates similar to Wolffian duct sperm, which is commonly used in sturgeon artificial propagation. Matured testicular sperm and Wolffian duct sperm post-thaw motility rate and curvilinear velocity were not significantly different, while duration of matured testicular sperm motility was significantly shorter than that of Wolffian duct sperm. Development rates of embryos obtained with post-thaw matured testicular sperm and Wolffian duct sperm were not significantly different. In vitro maturation of sterlet testicular sperm can potentially be useful in sperm cryobanking.
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