Alteration of thyroid hormone levels and related gene expression in Chinese rare minnow larvae exposed to mercury chloride
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25064382
DOI
10.1016/j.etap.2014.07.002
PII: S1382-6689(14)00156-2
Knihovny.cz E-resources
- Keywords
- Chinese rare minnow, Gene expression, Hypothalamic–pituitary–thyroid axis, Mercury, Thyroid hormone,
- MeSH
- Thyrotropin, beta Subunit genetics MeSH
- Water Pollutants, Chemical toxicity MeSH
- Mercuric Chloride toxicity MeSH
- Cyprinidae genetics metabolism MeSH
- Corticotropin-Releasing Hormone genetics MeSH
- Larva drug effects genetics metabolism MeSH
- Lethal Dose 50 MeSH
- Gene Expression Regulation drug effects MeSH
- Symporters genetics MeSH
- Thyroglobulin genetics MeSH
- Thyroxine metabolism MeSH
- Triiodothyronine metabolism MeSH
- Thyroid Hormone Receptors alpha genetics MeSH
- Thyroid Hormone Receptors beta genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Thyrotropin, beta Subunit MeSH
- Water Pollutants, Chemical MeSH
- Mercuric Chloride MeSH
- Corticotropin-Releasing Hormone MeSH
- sodium-iodide symporter MeSH Browser
- Symporters MeSH
- Thyroglobulin MeSH
- Thyroxine MeSH
- Triiodothyronine MeSH
- Thyroid Hormone Receptors alpha MeSH
- Thyroid Hormone Receptors beta MeSH
Mercury is a prominent environmental contaminant that causes endocrine disorder to human and other organisms. But little is known about the response of the thyroid functions and hypothalamic-pituitary-thyroid (HPT) axis to mercury in teleosts and the few studies that are available have not yielded consistent results. In this study, expression profiles of corticotropin-releasing hormone (crh), thyroid stimulating hormone beta (tshβ), solute carrier family 5 (sodium iodide symporter) member 5 (slc5a5), thyroglobulin (tg), thyroid hormone receptor alpha (trα) and thyroid hormone receptor beta (trβ) genes were determined in whole-body of Chinese rare minnow (Gobiocypris rarus) larvae after exposure to different levels of Hg(2+) (0, 0.1 and 0.3 mg/l) for 4 days, as well as the thyroid hormones (THs) levels. Moreover, the 96-h lethal concentration of Hg(2+) on rare minnow larvae was determined as 0.32 mg/l. The results showed that crh, tg, trα and trβ mRNA levels were significantly up-regulated in the larvae, but the gene expression of tshβ and slc5a5 was not significantly changed in our study. Besides, the THs levels increased in the whole-body of fish, especially the thyroxine (T4) level. The above results indicated that Hg(2+) could alter some genes expression in the HPT axis which could be used as the potential biomarkers for evaluating the environmental Hg(2+)-induced stress in fish.
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