Microscopy assays for evaluation of mast cell migration and chemotaxis
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Migration Assays methods MeSH
- Cell Culture Techniques instrumentation methods MeSH
- Cell Tracking methods MeSH
- Chemotactic Factors pharmacology MeSH
- Chemotaxis MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Mast Cells cytology drug effects physiology MeSH
- Microscopy methods MeSH
- Cell Movement * drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chemotactic Factors MeSH
A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.
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