Ménage-à-trois: the amoeba Nuclearia sp. from Lake Zurich with its ecto- and endosymbiotic bacteria
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25248027
DOI
10.1016/j.protis.2014.08.004
PII: S1434-4610(14)00084-4
Knihovny.cz E-resources
- Keywords
- Bacteria-protist symbioses, Nucleariidae, Paucibacter toxinivorans., ectosymbionts, endosymbionts, feeding,
- MeSH
- Amoeba classification cytology isolation & purification microbiology MeSH
- Betaproteobacteria classification isolation & purification physiology MeSH
- DNA, Bacterial chemistry genetics MeSH
- Gammaproteobacteria classification isolation & purification physiology MeSH
- Genes, rRNA MeSH
- Lakes parasitology MeSH
- DNA, Ribosomal Spacer chemistry genetics MeSH
- Molecular Sequence Data MeSH
- DNA, Protozoan chemistry genetics MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Protozoan genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- RNA, Ribosomal, 18S genetics MeSH
- Sequence Analysis, DNA MeSH
- Symbiosis * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Switzerland MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA, Ribosomal Spacer MeSH
- DNA, Protozoan MeSH
- DNA, Ribosomal MeSH
- RNA, Protozoan MeSH
- RNA, Ribosomal, 16S MeSH
- RNA, Ribosomal, 18S MeSH
We present a fascinating triad relationship between a eukaryotic amoeba and its two bacterial symbionts. The morphological characteristics of the amoeba allowed for a confident assignment to the genus Nuclearia (Opisthokonta, Nucleariidae), but species identification resulted in an ambiguous result. Sequence analysis indicated an affiliation to the species N. thermophila, however, several morphological features contradict the original description. Amoebal isolates were cultured for several years with their preferred food source, the microcystin-producing harmful cyanobacterium Planktothrix rubescens. Symbioses of the amoeba with ecto- and endosymbiotic bacteria were maintained over this period. Several thousand cells of the ectosymbiont are regularly arranged inside a layer of extracellular polymeric substances produced by the amoeba. The ectosymbiont was identified as Paucibacter toxinivorans (Betaproteobacteria), which was originally isolated by enrichment with microcystins. We found indications that our isolated ectosymbiont indeed contributed to toxin-degradation. The endosymbiont (Gammaproteobacteria, 15-20 bacteria per amoeba) is enclosed in symbiosomes inside the host cytoplasm and represents probably an obligate symbiont. We propose the name "Candidatus Endonucleariobacter rarus" for this bacterium that was neither found free-living nor in a symbiotic association. Nucleariidae are uniquely suited model organisms to study the basic principles of symbioses between opisthokonts and prokaryotes.
References provided by Crossref.org
CARD-FISH in the Sequencing Era: Opening a New Universe of Protistan Ecology
Life Cycle, Ultrastructure, and Phylogeny of New Diplonemids and Their Endosymbiotic Bacteria
GENBANK
HG530231, HG530232, HG530233, HG530234, HG530235, HG530236, HG530237, HG530238, HG530239, HG530240, HG530241, HG530242, HG530243, HG530244, HG530245, HG530246, HG530247, HG530248, HG530249, HG530250, HG530251, HG530252, HG530253, HG973425, HG973426, HG973427, HG973428, HG973429, HG973430, HG973431, HG973432, HG973433, HG973434, HG973435, HG973436, HG973437, HG973438, HG973439, HG973440, HG973441, HG973442, HG973443, HG973444, HG973445, HG973446, HG973447, HG973448, HG973449
