Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro
Language English Country Czech Republic Media print-electronic
Document type Journal Article
PubMed
25277490
DOI
10.5507/bp.2014.045
Knihovny.cz E-resources
- Keywords
- biocompatibility, human amniotic membrane, mesenchymal stem cells, quantum dots,
- MeSH
- Amnion cytology MeSH
- Staining and Labeling methods MeSH
- Biocompatible Materials metabolism MeSH
- Biological Assay methods MeSH
- Microscopy, Fluorescence methods MeSH
- Quantum Dots adverse effects metabolism MeSH
- Humans MeSH
- Mesenchymal Stem Cells metabolism MeSH
- In Vitro Techniques MeSH
- Materials Testing MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biocompatible Materials MeSH
BACKGROUND AND AIM: Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) are a potential source of mesenchymal stem cells which could be used to repair skin damage. The use of mesenchymal stem cells to repair skin damage requires safe, effective and biocompatible agents to evaluate the effectiveness of the result. Quantum dots (QDs) composed of CdSe/ZnS are semiconductor nanocrystals with broad excitation and narrow emission spectra, which have been considered as a new chemical and fluorescent substance for non-invasively labeling different cells in vitro and in vivo. This study investigated the cytotoxic effects of QDs on hAM-dMSCs at different times following labeling. METHODS: Using 0.75, 1.5 and 3.0 μL between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers. RESULTS: Quantum dot concentration 0.75 μg/mL labeled under a fluorescence microscope, cell morphology was observed, The MTT assay showed cells in the proliferative phase. Flow cytometry expression CD29, CD31, CD34, CD44, CD90, CD105 and CD106. CONCLUSIONS: Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility.
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