Diamond nanoparticles (DNPs) are very attractive for biomedical applications, particularly for bioimaging. The aim of this study was to evaluate the impact of DNPs on neural cancer cells and thus to assess the possible application of DNPs for these cells imaging. For this purpose, the neuroblastoma SH-SY5Y cell line was chosen. Cells were cultured in medium with different concentrations (15, 50, 100 and 150 μg/ml) of DNPs. After 48 h of incubation, cell metabolic activity was evaluated by the XTT assay. For assessment of cellular metabolic activity, cells were also cultured on differently terminated nanocrystalline diamond (NCD) coatings in medium with 150 μg/ml of DNPs. Cell adhesion and morphology were evaluated by brightfield microscopy. Diamond nanoparticle internalization was determined by confocal microscopy. The obtained results showed that low concentrations (15, 50 and 100 μg/ml) of nanoparticles did not significantly affect the SH-SY5Y cell metabolic activity. However, a higher concentration (150 μg/ml) of DNPs statistically significantly reduced SH-SY5Y cell metabolic activity. After 48 h incubation with 150 μg/ml DNPs, cell metabolic activity was 23% lower than in medium without DNPs on standard tissue culture polystyrene.

Czech Technical University Prague Faculty of Biomedical Engineering Sítná sq 3105 272 01 Kladno Czech Republic

Department of Physical Chemistry Faculty of Chemistry Vilnius University Naugarduko 24 LT 03225 Vilnius Lithuania

Department of Physical Chemistry Faculty of Chemistry Vilnius University Naugarduko 24 LT 03225 Vilnius Lithuania; Department of Stem Cell Biology State Research Institute Centre for Innovative Medicine Zygimantu 9 LT 01102 Vilnius Lithuania

Department of Physical Chemistry Faculty of Chemistry Vilnius University Naugarduko 24 LT 03225 Vilnius Lithuania; Laboratory of NanoBioTechnology Department of Materials Science and Electronics Institute of Semiconductor Physics State Scientific Research Institute Centre for Physical Sciences and Technology A Gostauto 11 LT 01108 Vilnius Lithuania

Department of Stem Cell Biology State Research Institute Centre for Innovative Medicine Zygimantu 9 LT 01102 Vilnius Lithuania

Hasselt University Biomedical Research Institute Agoralaan Building C Diepenbeek B 3590 Belgium

Hasselt University Biomedical Research Institute Agoralaan Building C Diepenbeek B 3590 Belgium; Hasselt University Institute for Materials Research Division IMOMEC Wetenschapspark 1 B 3590 Diepenbeek Belgium

Hasselt University Institute for Materials Research Division IMOMEC Wetenschapspark 1 B 3590 Diepenbeek Belgium

NanoTechnas Center of Nanotechnology and Material Science Faculty of Chemistry Vilnius University Naugarduko 24 LT 03225 Vilnius Lithuania

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