A method for determining supernatant-free spectra generated from fluorescently stained cells in suspension
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25463661
DOI
10.1016/j.jphotobiol.2014.10.006
PII: S1011-1344(14)00303-0
Knihovny.cz E-resources
- MeSH
- Spectrometry, Fluorescence * instrumentation MeSH
- Carbocyanines chemistry MeSH
- Membrane Potentials MeSH
- Saccharomyces cerevisiae cytology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3,3'-dipropylthiacarbocyanine MeSH Browser
- Carbocyanines MeSH
Here we present a fluorometric method for direct determination of supernatant-free fluorescence spectra generated from fluorescently stained cells in suspension. The key element in the new technique is the design of an adapter to a standard cuvette holder that makes it possible to measure front-face fluorescence spectra from thin layers of cells spun down to the bottom of a spectrofluorometric cuvette. We have demonstrated the applicability of this approach and its analytical potential using the suspensions of yeast cells stained with the potentiometric dye of 3,3'-dipropylthiadicarbocyanine, diS-C3(3), and with the specific cell-wall marker calcofluor.
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