Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometry
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25465366
DOI
10.1016/j.chroma.2014.11.015
PII: S0021-9673(14)01773-7
Knihovny.cz E-resources
- Keywords
- ESI–MS, HILIC separation, Monolithic silica column, Nucleobase, Nucleoside, Nucleotide,
- MeSH
- Spectrometry, Mass, Electrospray Ionization instrumentation methods MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Nucleosides chemistry isolation & purification MeSH
- Nucleotides chemistry isolation & purification MeSH
- Silicon Dioxide chemistry MeSH
- Surface Properties MeSH
- Reproducibility of Results MeSH
- Tandem Mass Spectrometry instrumentation methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nucleosides MeSH
- Nucleotides MeSH
- Silicon Dioxide MeSH
The capability of employing synthesized zwitterionic silica-based monolithic capillary columns (140 mm × 0.1mm) for separation of highly polar and hydrophilic nucleobases, nucleosides, and nucleotides in hydrophilic interaction chromatography is reported. The suitability of the columns for on-line conjunction with electrospray tandem mass spectrometry was explored. Our results show that the grafted layer of zwitterionic monomer ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide or 2-methacryloyloxyethyl phosphorylcholine) on the silica monolithic surface significantly improved the separation selectivity and reproducibility, as compared to the bare silica monolith. The stepwise elution from 90% to 70% of acetonitrile enabled separation of a complex sample mixture containing 21 compounds with a total analysis time less than 40 min.
Department of Chemistry P O Box 55 00014 University of Helsinki Finland
Faculty of Pharmacy P O Box 56 00014 University of Helsinki Finland
Institute of Analytical Chemistry of the ASCR v v i Veveří 97 60200 Brno Czech Republic
Steno Diabetes Center Niels Steensens Vej 2 2820 Gentofte Denmark
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